abstract：:Ubiquitination refers to the covalent addition of ubiquitin (Ub) to substrate proteins or other Ub molecules via the sequential action of three enzymes (E1, E2, and E3). Recent advances in mass spectrometry proteomics have made it possible to identify and quantify Ub linkages in biochemical and cellular systems. We us...

journal_title：Journal of molecular biology

authors： Bosanac I,Phu L,Pan B,Zilberleyb I,Maurer B,Dixit VM,Hymowitz SG,Kirkpatrick DS

更新日期：2011-05-06 00:00:00

abstract：:Hookworms are human parasites that have devastating effects on global health, particularly in underdeveloped countries. Ancylostoma ceylanicum infects humans and animals, making it a useful model organism to study disease pathogenesis. A. ceylanicum excretory-secretory protein 2 (AceES-2), a highly immunoreactive mole...

journal_title：Journal of molecular biology

authors： Kucera K,Harrison LM,Cappello M,Modis Y

更新日期：2011-04-22 00:00:00

abstract：:Simocyclinone D8 (SD8), a potent DNA gyrase inhibitor made by Streptomyces antibioticus, is exported from the producing organism by the SimX efflux pump. The expression of simX is under the control of SimR, a member of the TetR family of transcriptional regulators. SimR represses simX transcription by binding to opera...

journal_title：Journal of molecular biology

authors： Le TB,Stevenson CE,Fiedler HP,Maxwell A,Lawson DM,Buttner MJ

更新日期：2011-04-22 00:00:00

abstract：:Biochemical functions of proteins in cells frequently involve interactions with various ligands. Proteomic methods for the identification of proteins that interact with specific ligands such as metabolites, signaling molecules, and drugs are valuable in investigating the regulatory mechanisms of cellular metabolism, a...

journal_title：Journal of molecular biology

authors： Liu PF,Kihara D,Park C

更新日期：2011-04-22 00:00:00

abstract：:Reverse transcriptases (RTs) encoded by a wide range of mobile retroelements have had a major impact on the structure and function of genomes. Among the most abundant elements in eukaryotes are the non long terminal repeat (LTR) retrotransposons. Here we compare the dNTP concentration requirements and error rates of t...

journal_title：Journal of molecular biology

authors： Jamburuthugoda VK,Eickbush TH

更新日期：2011-04-15 00:00:00

abstract：:The cellulosome is a highly elaborate cell-bound multienzyme complex that efficiently orchestrates the deconstruction of cellulose and hemicellulose, two of the nature's most abundant polymers. Understanding the intricacy of these nanomachines evolved by anaerobic microbes could sustain the development of an effective...

journal_title：Journal of molecular biology

authors： García-Alvarez B,Melero R,Dias FM,Prates JA,Fontes CM,Smith SP,Romão MJ,Carvalho AL,Llorca O

更新日期：2011-04-08 00:00:00

abstract：:Mutations in CAPN3/Capn3, which codes for skeletal muscle-specific calpain-3/p94 protease, are responsible for limb-girdle muscular dystrophy type 2A. Using "knock-in" (referred to as Capn3(CS/CS)) mice, in which the endogenous calpain-3 is replaced with a mutant calpain-3:C129S, which is a proteolytically inactive bu...

journal_title：Journal of molecular biology

authors： Ojima K,Ono Y,Ottenheijm C,Hata S,Suzuki H,Granzier H,Sorimachi H

更新日期：2011-04-01 00:00:00

abstract：:Although it has been known for many years that antibodies display properties characteristic of allosteric effectors, the molecular mechanisms responsible for these effects remain poorly understood. Here, we describe a single-domain antibody fragment (nanobody) that modulates protein function by constraining conformati...

journal_title：Journal of molecular biology

authors： Oyen D,Srinivasan V,Steyaert J,Barlow JN

更新日期：2011-03-18 00:00:00

abstract：:14-3-3 proteins participate in many key cellular processes after binding to disordered phospho-partners. Usually, the phosphorylated state is an essential target for the binding. Here, we show for the first time residues other than those in the 14-3-3 binding motif that are essential for the binding between 14-3-3 and...

journal_title：Journal of molecular biology

authors： Uhart M,Iglesias AA,Bustos DM

更新日期：2011-03-04 00:00:00

abstract：:The capsid (CA) protein plays crucial roles in HIV infection and replication, essential to viral maturation. The absence of high-resolution structural data on unassembled CA hinders the development of antivirals effective in inhibiting assembly. Unlike enzymes that have targetable, functional substrate-binding sites, ...

journal_title：Journal of molecular biology

authors： Du S,Betts L,Yang R,Shi H,Concel J,Ahn J,Aiken C,Zhang P,Yeh JI

更新日期：2011-02-25 00:00:00

abstract：:Enoyl-[acyl carrier protein] (ACP) reductase (ENR) is a key enzyme in type II fatty acid synthesis that catalyzes the last step in each elongation cycle. Therefore, it has been considered as a target for antibiotics. However, recent studies indicate that some pathogens have more than one ENR; in particular, Bacillus s...

journal_title：Journal of molecular biology

authors： Kim KH,Ha BH,Kim SJ,Hong SK,Hwang KY,Kim EE

更新日期：2011-02-25 00:00:00

abstract：:Previous work has shown that the α-tocopherol transfer protein (α-TTP) can bind to vesicular or immobilized phospholipid membranes. Revealing the molecular mechanisms by which α-TTP associates with membranes is thought to be critical to understanding its function and role in the secretion of tocopherol from hepatocyte...

journal_title：Journal of molecular biology

authors： Zhang WX,Thakur V,Lomize A,Pogozheva I,Panagabko C,Cecchini M,Baptist M,Morley S,Manor D,Atkinson J

更新日期：2011-01-28 00:00:00

abstract：:Aminoacyl-tRNA synthetases are validated molecular targets for anti-infective drug discovery because of their essentiality in protein synthesis. Thanks to genome sequencing, it is now possible to systematically study aminoacyl-tRNA synthetases from human eukaryotic parasites as putative targets for novel drug discover...

journal_title：Journal of molecular biology

authors： Crepin T,Peterson F,Haertlein M,Jensen D,Wang C,Cusack S,Kron M

更新日期：2011-01-28 00:00:00

abstract：:Bacteriophages are involved in many aspects of the spread and establishment of virulence factors in Staphylococcus aureus, including the mobilization of genetic elements known as S. aureus pathogenicity islands (SaPIs), which carry genes for superantigen toxins and other virulence factors. SaPIs are packaged into phag...

journal_title：Journal of molecular biology

authors： Spilman MS,Dearborn AD,Chang JR,Damle PK,Christie GE,Dokland T

更新日期：2011-01-21 00:00:00

abstract：:Phospholamban physically interacts with the sarcoplasmic reticulum calcium pump (SERCA) and regulates contractility of the heart in response to adrenergic stimuli. We studied this interaction using electron microscopy of 2D crystals of SERCA in complex with phospholamban. In earlier studies, phospholamban oligomers we...

journal_title：Journal of molecular biology

authors： Glaves JP,Trieber CA,Ceholski DK,Stokes DL,Young HS

更新日期：2011-01-21 00:00:00

abstract：:Drugs can affect function in proteins by modulating their flexibility. Despite this possibility, there are very few studies on how drug binding affects the dynamics of target macromolecules. FKBP12 (FK506 binding protein 12) is a prolyl cis-trans isomerase and a drug target. The immunosuppressant drug rapamycin exerts...

journal_title：Journal of molecular biology

authors： Sapienza PJ,Mauldin RV,Lee AL

更新日期：2011-01-14 00:00:00

abstract：:Physarum polycephalum hemagglutinin I (HA1) is a 104-residue protein that is secreted to extracellular space. The crystal structure of HA1 has a β-sandwich fold found among lectin structures, such as legume lectins and galectins. Interestingly, the β-sandwich of HA1 lacks a jelly roll motif and is essentially composed...

journal_title：Journal of molecular biology

authors： Kouno T,Watanabe N,Sakai N,Nakamura T,Nabeshima Y,Morita M,Mizuguchi M,Aizawa T,Demura M,Imanaka T,Tanaka I,Kawano K

更新日期：2011-01-14 00:00:00

abstract：:The 37/ 67-kDa human laminin receptor (LamR) is a cell surface receptor for laminin, prion protein, and a variety of viruses. Because of its wide range of ligands, LamR plays a role in numerous pathologies. LamR overexpression correlates with a highly invasive cell phenotype and increased metastatic ability, mediated ...

journal_title：Journal of molecular biology

authors： Jamieson KV,Hubbard SR,Meruelo D

更新日期：2011-01-07 00:00:00

abstract：:A 26 Å resolution map of the structure of human low-density lipoprotein (LDL) was obtained from electron cryomicroscopy and single-particle image reconstruction. The structure showed a discoidal-shaped LDL particle with high-density regions mainly distributed at the edge of the particle and low-density regions at the ...

journal_title：Journal of molecular biology

authors： Liu Y,Atkinson D

更新日期：2011-01-07 00:00:00

abstract：:Myosin VI is a molecular motor that is thought to function both as a transporter and as a cytoskeletal anchor in vivo. Here we use optical tweezers to examine force generation by single molecules of myosin VI under physiological nucleotide concentrations. We find that myosin VI is an efficient transporter at loads of ...

journal_title：Journal of molecular biology

authors： Chuan P,Spudich JA,Dunn AR

更新日期：2011-01-07 00:00:00

abstract：:Spiders that spin orb webs secrete seven types of silk. Although the spinning process of the dragline thread is beginning to be understood, the molecular events that occur in spiders' opisthosomal glands, which produce the other fibers, are unknown due to a lack of data regarding their initial and final structures. Ta...

journal_title：Journal of molecular biology

authors： Lefèvre T,Boudreault S,Cloutier C,Pézolet M

更新日期：2011-01-07 00:00:00

abstract：:The phycobilisome light-harvesting antenna in cyanobacteria and red algae is assembled from two substructures: a central core composed of allophycocyanin surrounded by rods that always contain phycocyanin (PC). Unpigmented proteins called linkers are also found within the rods and core. We present here two new structu...

journal_title：Journal of molecular biology

authors： David L,Marx A,Adir N

更新日期：2011-01-07 00:00:00

abstract：:Isoprenoids, most of them synthesized by prenyltransferases (PTSs), are a class of important biologically active compounds with diverse functions. The mint geranyl pyrophosphate synthase (GPPS) is a heterotetramer composed of two LSU·SSU (large/small subunit) dimers. In addition to C(10)-GPP, the enzyme also produces ...

journal_title：Journal of molecular biology

authors： Hsieh FL,Chang TH,Ko TP,Wang AH

更新日期：2010-12-17 00:00:00

abstract：:CloQ is an aromatic prenyltransferase from the clorobiocin biosynthetic pathway of Streptomyces roseochromogenes var. oscitans. It is involved in the synthesis of the prenylated hydroxybenzoate moiety of the antibiotic, specifically catalyzing the attachment of a dimethylallyl moiety to 4-hydroxyphenylpyruvate. Herein...

journal_title：Journal of molecular biology

authors： Metzger U,Keller S,Stevenson CE,Heide L,Lawson DM

更新日期：2010-12-10 00:00:00

abstract：:The interactions between the complement receptor type 2 (CR2) and the C3 complement fragments C3d, C3dg, and iC3b are essential for the initiation of a normal immune response. A crystal-derived structure of the two N-terminal short consensus repeat (SCR1-2) domains of CR2 in complex with C3d has previously been elucid...

journal_title：Journal of molecular biology

authors： Shaw CD,Storek MJ,Young KA,Kovacs JM,Thurman JM,Holers VM,Hannan JP

更新日期：2010-12-10 00:00:00

abstract：:Anterior gradient-2 (AGR2) functions in a range of biological systems, including goblet cell formation, limb regeneration, inhibition of p53, and metastasis. There are no well-validated binding proteins for AGR2 protein despite the wealth of data implicating an important cellular function in vertebrates. The yeast two...

journal_title：Journal of molecular biology

authors： Maslon MM,Hrstka R,Vojtesek B,Hupp TR

更新日期：2010-12-03 00:00:00

abstract：:Rvb1 and Rvb2 are essential AAA(+) (ATPases associated with diverse cellular activities) helicases, which are important components of critical complexes such as chromatin remodeling and telomerase complexes. The oligomeric state of the Rvb proteins has been controversial. Independent studies from several groups have d...

journal_title：Journal of molecular biology

authors： Cheung KL,Huen J,Kakihara Y,Houry WA,Ortega J

更新日期：2010-12-03 00:00:00

abstract：:Spider dragline silk, one of the strongest polymers in nature, is composed of proteins termed major ampullate spidroin (MaSp) 1 and MaSp2. The N-terminal (NT) domain of MaSp1 produced by the nursery web spider Euprosthenops australis acts as a pH-sensitive relay, mediating spidroin assembly at around pH 6.3. Using ami...

journal_title：Journal of molecular biology

authors： Landreh M,Askarieh G,Nordling K,Hedhammar M,Rising A,Casals C,Astorga-Wells J,Alvelius G,Knight SD,Johansson J,Jörnvall H,Bergman T

更新日期：2010-11-26 00:00:00

abstract：:A number of approaches have been utilized to generate antibodies to cancer cell surface receptors that can be used as potential therapeutics. A number of these therapeutic approaches, including antibody-drug conjugates, immunotoxins, and targeted nucleic acid delivery, require antibodies that not only bind receptor bu...

journal_title：Journal of molecular biology

authors： Zhou Y,Zou H,Zhang S,Marks JD

更新日期：2010-11-19 00:00:00

abstract：:The outer membrane usher protein Caf1A of the plague pathogen Yersinia pestis is responsible for the assembly of a major surface antigen, the F1 capsule. The F1 capsule is mainly formed by thin linear polymers of Caf1 (capsular antigen fraction 1) protein subunits. The Caf1A usher promotes polymerization of subunits a...

journal_title：Journal of molecular biology

authors： Dubnovitsky AP,Duck Z,Kersley JE,Härd T,MacIntyre S,Knight SD

更新日期：2010-10-22 00:00:00

abstract：:X-ray crystallography and NMR can provide detailed structural information of protein-protein complexes, but technical problems make their application challenging in the high-throughput regime. Other methods such as small-angle X-ray scattering (SAXS) are more promising for large-scale application, but at the cost of l...

journal_title：Journal of molecular biology

authors： Pons C,D'Abramo M,Svergun DI,Orozco M,Bernadó P,Fernández-Recio J

更新日期：2010-10-22 00:00:00

abstract：:The feline immunodeficiency virus (FIV) is a lentivirus that is related to human immunodeficiency virus (HIV), causing a similar pathology in cats. It is a potential small animal model for AIDS and the FIV-based vectors are also being pursued for human gene therapy. Previous studies have mapped the FIV packaging signa...

journal_title：Journal of molecular biology

authors： Rizvi TA,Kenyon JC,Ali J,Aktar SJ,Phillip PS,Ghazawi A,Mustafa F,Lever AML

更新日期：2010-10-15 00:00:00

abstract：:Recombinant expression systems differ in the type of glycosylation they impart on expressed antigens such as the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins, potentially affecting their biological properties. We performed head-to-head antigenic, immunogenic and molecular profiling of two distant...

journal_title：Journal of molecular biology

authors： Kong L,Sheppard NC,Stewart-Jones GBE,Robson CL,Chen H,Xu X,Krashias G,Bonomelli C,Scanlan CN,Kwong PD,Jeffs SA,Jones IM,Sattentau QJ

更新日期：2010-10-15 00:00:00

abstract：:Fish hatching enzymes are zinc metalloproteases that digest the egg envelope (chorion) at the time of hatching. The crystal structure of zebrafish hatching enzyme 1 (ZHE1) has been solved at 1.10 Å resolution. ZHE1 is monomeric, is mitten shaped, and has a cleft at the center of the molecule. ZHE1 consists of three 3(...

journal_title：Journal of molecular biology

authors： Okada A,Sano K,Nagata K,Yasumasu S,Ohtsuka J,Yamamura A,Kubota K,Iuchi I,Tanokura M

更新日期：2010-10-08 00:00:00

abstract：:Interleukin (IL)-12 and IL-23 are heterodimeric proinflammatory cytokines that share a common p40 subunit, paired with p35 and p19 subunits, respectively. They represent an attractive class of therapeutic targets for the treatment of psoriasis and other immune-mediated diseases. Ustekinumab is a fully human monoclonal...

journal_title：Journal of molecular biology

authors： Luo J,Wu SJ,Lacy ER,Orlovsky Y,Baker A,Teplyakov A,Obmolova G,Heavner GA,Richter HT,Benson J

更新日期：2010-10-08 00:00:00

abstract：:The small GTPases Arf1 and Arf6 have nonoverlapping functions in cellular traffic despite their very high sequence and structural resemblance. Notably, the exquisite isoform specificity of their guanine nucleotide exchange factors and their distinctive sensitivity to the drug brefeldin A cannot be explained by any str...

journal_title：Journal of molecular biology

authors： Biou V,Aizel K,Roblin P,Thureau A,Jacquet E,Hansson S,Guibert B,Guittet E,van Heijenoort C,Zeghouf M,Perez J,Cherfils J

更新日期：2010-10-01 00:00:00

abstract：:DEAD box proteins consist of a common helicase core formed by two globular RecA domains that are separated by a cleft. The helicase core acts as a nucleotide-dependent switch that alternates between open and closed conformations during the catalytic cycle of duplex separation, thereby providing basic helicase activity...

journal_title：Journal of molecular biology

authors： Karow AR,Klostermeier D

更新日期：2010-10-01 00:00:00

abstract：:Protein folding involves the formation of secondary structural elements from the primary sequence and their association with tertiary assemblies. The relation of this primary sequence to a specific folded protein structure remains a central question in structural biology. An increasing body of evidence suggests that v...

journal_title：Journal of molecular biology

authors： Schellenberg MJ,Ritchie DB,Wu T,Markin CJ,Spyracopoulos L,MacMillan AM

更新日期：2010-10-01 00:00:00

abstract：:Alphavirus replicons are very useful for analyzing different aspects of viral molecular biology. They are also useful tools in the development of new vaccines and highly efficient expression of heterologous genes. We have investigated the translatability of Sindbis virus (SV) subgenomic mRNA bearing different 5'-untra...

journal_title：Journal of molecular biology

authors： Sanz MA,Welnowska E,Redondo N,Carrasco L

更新日期：2010-09-10 00:00:00

abstract：:l-Gulonate 3-dehydrogenase (GDH) is a bifunctional dimeric protein that functions not only as an NAD(+)-dependent enzyme in the uronate cycle but also as a taxon-specific lambda-crystallin in rabbit lens. Here we report the first crystal structure of GDH in both apo form and NADH-bound holo form. The GDH protomer cons...

journal_title：Journal of molecular biology

authors： Asada Y,Kuroishi C,Ukita Y,Sumii R,Endo S,Matsunaga T,Hara A,Kunishima N

更新日期：2010-09-03 00:00:00